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eLife Volume 9 ,2020-12-10
A gustatory receptor tuned to the steroid plant hormone brassinolide in Plutella xylostella (Lepidoptera: Plutellidae)
Ke Yang 1 , 2 Xin-Lin Gong 1 , 2 Guo-Cheng Li 1 , 2 Ling-Qiao Huang 1 Chao Ning 1 , 2 Chen-Zhu Wang 1 , 2
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Received 2020-10-17, accepted for publication 2020-12-10, Published 2020-12-10

Feeding and oviposition deterrents help phytophagous insects to identify host plants. The taste organs of phytophagous insects contain bitter gustatory receptors (GRs). To explore their function, the GRs in Plutella xylostella were analyzed. Through RNA sequencing and qPCR, we detected abundant PxylGr34 transcripts in the larval head and adult antennae. Functional analyses using the Xenopus oocyte expression system and 24 diverse phytochemicals showed that PxylGr34 is tuned to the canonical plant hormones brassinolide (BL) and 24-epibrassinolide (EBL). Electrophysiological analyses revealed that the medial sensilla styloconica of 4th instar larvae are responsive to BL and EBL. Dual-choice bioassays demonstrated that BL inhibits larval feeding and female oviposition. Knock-down of PxylGr34 by RNAi attenuates the taste responses to BL, and abolishes BL-induced feeding inhibition. These results increase our understanding of how herbivorous insects detect compounds that deter feeding and oviposition, and may be useful for designing plant hormone-based pest management strategies.


Other;oviposition bioassays;feeding;brassinolide;RNAi;functional analysis;gustatory receptor;Plutella xylostella


© 2020, Yang et al
http://creativecommons.org/licenses/by/4.0/This article is distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use and redistribution provided that the original author and source are credited.


Ke Yang,Xin-Lin Gong,Guo-Cheng Li,Ling-Qiao Huang,Chao Ning,Chen-Zhu Wang. A gustatory receptor tuned to the steroid plant hormone brassinolide in Plutella xylostella (Lepidoptera: Plutellidae). eLife ,Vol.9(2020)



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