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eLife Volume 9 ,2020-04-06
Condensin I subunit Cap-G is essential for proper gene expression during the maturation of post-mitotic neurons
Developmental Biology
Amira Hassan 1 Pablo Araguas Rodriguez 1 Stefan K Heidmann 2 Emma L Walmsley 1 Gabriel N Aughey 1 Tony D Southall 1
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DOI:10.7554/eLife.55159
Received 2020-01-14, accepted for publication 2020-04-06, Published 2020-04-06
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摘要

Condensin complexes are essential for mitotic chromosome assembly and segregation during cell divisions, however, little is known about their functions in post-mitotic cells. Here we report a role for the condensin I subunit Cap-G in Drosophila neurons. We show that, despite not requiring condensin for mitotic chromosome compaction, post-mitotic neurons express Cap-G. Knockdown of Cap-G specifically in neurons (from their birth onwards) results in developmental arrest, behavioural defects, and dramatic gene expression changes, including reduced expression of a subset of neuronal genes and aberrant expression of genes that are not normally expressed in the developing brain. Knockdown of Cap-G in mature neurons results in similar phenotypes but to a lesser degree. Furthermore, we see dynamic binding of Cap-G at distinct loci in progenitor cells and differentiated neurons. Therefore, Cap-G is essential for proper gene expression in neurons and plays an important role during the early stages of neuronal development.

关键词

D. melanogaster;DamID;post-mitotic;development;neuron;condensin

授权许可

© 2020, Hassan et al
http://creativecommons.org/licenses/by/4.0/This article is distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use and redistribution provided that the original author and source are credited.

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Amira Hassan,Pablo Araguas Rodriguez,Stefan K Heidmann,Emma L Walmsley,Gabriel N Aughey,Tony D Southall. Condensin I subunit Cap-G is essential for proper gene expression during the maturation of post-mitotic neurons. eLife ,Vol.9(2020)

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