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eLife Volume 9 ,2020-03-29
Increase of circulating IGFBP-4 following genotoxic stress and its implication for senescence
Human Biology and Medicine
Nicola Alessio 1 Tiziana Squillaro 1 Giovanni Di Bernardo 2 Giovanni Galano 2 Roberto De Rosa 2 Mariarosa AB Melone 3 Gianfranco Peluso 4 Umberto Galderisi 1 , 4 , 5
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DOI:10.7554/eLife.54523
Received 2019-12-17, accepted for publication 2020-03-29, Published 2020-03-29
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摘要

Senescent cells secrete several molecules, collectively named senescence-associated secretory phenotype (SASP). In the SASP of cells that became senescent following several in vitro chemical and physical stress, we identified the IGFBP-4 protein that can be considered a general stress mediator. This factor appeared to play a key role in senescence-paracrine signaling. We provided evidences showing that genotoxic injury, such as low dose irradiation, may promote an IGFBP-4 release in bloodstream both in mice irradiated with 100 mGy X-ray and in human subjects that received Computer Tomography. Increased level of circulating IGFBP-4 may be responsible of pro-aging effect. We found a significant increase of senescent cells in the lungs, heart, and kidneys of mice that were intraperitoneally injected with IGFBP-4 twice a week for two months. We then analyzed how genotoxic stressors may promote the release of IGFBP-4 and the molecular pathways associated with the induction of senescence by this protein.

关键词

Mouse;Human;secretome;SASP;senescence;paracrine signaling;mesenchymal stromal cells

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© 2020, Alessio et al
http://creativecommons.org/licenses/by/4.0/This article is distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use and redistribution provided that the original author and source are credited.

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Nicola Alessio,Tiziana Squillaro,Giovanni Di Bernardo,Giovanni Galano,Roberto De Rosa,Mariarosa AB Melone,Gianfranco Peluso,Umberto Galderisi. Increase of circulating IGFBP-4 following genotoxic stress and its implication for senescence. eLife ,Vol.9(2020)

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