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eLife Volume 9 ,2020-03-30
Intermediate progenitors support migration of neural stem cells into dentate gyrus outer neurogenic niches
Developmental Biology
Branden R Nelson 1 , 2 Rebecca D Hodge 1 Ray AM Daza 1 Prem Prakash Tripathi 1 Sebastian J Arnold 3 , 4 Kathleen J Millen 1 , 5 Robert F Hevner 1 , 2
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DOI:10.7554/eLife.53777
Received 2019-11-20, accepted for publication 2020-03-30, Published 2020-03-30
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摘要

The hippocampal dentate gyrus (DG) is a unique brain region maintaining neural stem cells (NCSs) and neurogenesis into adulthood. We used multiphoton imaging to visualize genetically defined progenitor subpopulations in live slices across key stages of mouse DG development, testing decades old static models of DG formation with molecular identification, genetic-lineage tracing, and mutant analyses. We found novel progenitor migrations, timings, dynamic cell-cell interactions, signaling activities, and routes underlie mosaic DG formation. Intermediate progenitors (IPs, Tbr2+) pioneered migrations, supporting and guiding later emigrating NSCs (Sox9+) through multiple transient zones prior to converging at the nascent outer adult niche in a dynamic settling process, generating all prenatal and postnatal granule neurons in defined spatiotemporal order. IPs (Dll1+) extensively targeted contacts to mitotic NSCs (Notch active), revealing a substrate for cell-cell contact support during migrations, a developmental feature maintained in adults. Mouse DG formation shares conserved features of human neocortical expansion.

关键词

Mouse;dentate gyrus;multiphoton live-imaging;delta - notch;neural stem cell;tbr2 eomes intermediate progenitor;neurogenesis;hippocampus

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© 2020, Nelson et al
http://creativecommons.org/licenses/by/4.0/This article is distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use and redistribution provided that the original author and source are credited.

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Branden R Nelson,Rebecca D Hodge,Ray AM Daza,Prem Prakash Tripathi,Sebastian J Arnold,Kathleen J Millen,Robert F Hevner. Intermediate progenitors support migration of neural stem cells into dentate gyrus outer neurogenic niches. eLife ,Vol.9(2020)

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