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eLife Volume 8 ,2019-11-19
Single cell transcriptome atlas of the Drosophila larval brain
Neuroscience
Clarisse Brunet Avalos 1 G Larisa Maier 1 Rémy Bruggmann 2 Simon G Sprecher 1
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DOI:10.7554/eLife.50354
Received 2019-07-19, accepted for publication 2019-11-19, Published 2019-11-19
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摘要

Cell diversity of the brain and how it is affected by starvation, remains largely unknown. Here, we introduce a single cell transcriptome atlas of the entire Drosophila first instar larval brain. We first assigned cell-type identity based on known marker genes, distinguishing five major groups: neural progenitors, differentiated neurons, glia, undifferentiated neurons and non-neural cells. All major classes were further subdivided into multiple subtypes, revealing biological features of various cell-types. We further assessed transcriptional changes in response to starvation at the single-cell level. While after starvation the composition of the brain remains unaffected, transcriptional profile of several cell clusters changed. Intriguingly, different cell-types show very distinct responses to starvation, suggesting the presence of cell-specific programs for nutrition availability. Establishing a single-cell transcriptome atlas of the larval brain provides a powerful tool to explore cell diversity and assess genetic profiles from developmental, functional and behavioral perspectives.

关键词

D. melanogaster;nervous system;cell diversity;brain;larva;Drosophila;single cell transcriptome

授权许可

© 2019, Brunet Avalos et al
http://creativecommons.org/licenses/by/4.0/This article is distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use and redistribution provided that the original author and source are credited.

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Clarisse Brunet Avalos,G Larisa Maier,Rémy Bruggmann,Simon G Sprecher. Single cell transcriptome atlas of the Drosophila larval brain. eLife ,Vol.8(2019)

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