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Dose-Response Volume 17 ,Issue 3 ,2019-09-25
Genome-Wide Transcriptome Analysis Reveals Intermittent Fasting-Induced Metabolic Rewiring in the Liver
Original Article
Gavin Yong-Quan Ng 1 Sung-Wook Kang 1 Joonki Kim 1 , 2 Asfa Alli-Shaik 3 Sang-Ha Baik 1 Dong-Gyu Jo 4 M. Prakash Hande 1 Christopher G. Sobey 5 Jayantha Gunaratne 3 , 6 David Yang-Wei Fann 1 Thiruma V. Arumugam 1 , 7 , 8
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Received 2019-4-27, accepted for publication 2019-8-27, Published 2019-8-27

Scope: Intermittent fasting (IF) has been extensively reported to promote improved energy homeostasis and metabolic switching. While IF may be a plausible strategy to ameliorate the epidemiological burden of disease in many societies, our understanding of the underlying molecular mechanisms behind such effects is still lacking. The present study has sought to investigate the relationship between IF and changes in gene expression. We focused on the liver, which is highly sensitive to metabolic changes due to energy status. Mice were randomly assigned to ad libitum feeding or IF for 16 hours per day or for 24 hours on alternate days for 3 months, after which genome-wide transcriptome analysis of the liver was performed using RNA sequencing. Our findings revealed that IF caused robust transcriptomic changes in the liver that led to a complex array of metabolic changes. We also observed that the IF regimen produced distinct profiles of transcriptomic changes, highlighting the significance of temporally different periods of energy restriction. Our results suggest that IF can regulate metabolism via transcriptomic mechanisms and provide insight into how genetic interactions within the liver might lead to the numerous metabolic benefits of IF.


RNA sequencing;intermittent fasting;liver;metabolism;transcriptome


© The Author(s) 2019
This article is distributed under the terms of the Creative Commons Attribution-NonCommercial 4.0 License (http://www.creativecommons.org/licenses/by-nc/4.0/) which permits non-commercial use, reproduction and distribution of the work without further permission provided the original work is attributed as specified on the SAGE and Open Access pages (https://us.sagepub.com/en-us/nam/open-access-at-sage).


1. David Yang-Wei Fann.Department of Physiology, Yong Loo Lin School of Medicine, National University of Singapore, Singapore, Singapore.phstva@nus.edu.sg
2. Thiruma V. Arumugam.Department of Physiology, Yong Loo Lin School of Medicine, National University of Singapore, Singapore, Singapore; School of Pharmacy, Sungkyunkwan University, Suwon, Republic of Korea; Neurobiology Programme, Life Sciences Institute, National University of Singapore, Singapore, Singapore.phstva@nus.edu.sg


Gavin Yong-Quan Ng,Sung-Wook Kang,Joonki Kim,Asfa Alli-Shaik,Sang-Ha Baik,Dong-Gyu Jo,M. Prakash Hande,Christopher G. Sobey,Jayantha Gunaratne,David Yang-Wei Fann,Thiruma V. Arumugam. Genome-Wide Transcriptome Analysis Reveals Intermittent Fasting-Induced Metabolic Rewiring in the Liver. Dose-Response ,Vol.17, Issue 3(2019)



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