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Oxidative Medicine and Cellular Longevity Volume 2019 ,2019-03-06
Epicatechin Gallate Protects HBMVECs from Ischemia/Reperfusion Injury through Ameliorating Apoptosis and Autophagy and Promoting Neovascularization
Research Article
Bing Fu 1 Qinghong Zeng 1 Zhaoting Zhang 1 Mingyue Qian 1 Jiechun Chen 1 Wanli Dong 2 Min Li 1
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DOI:10.1155/2019/7824684
Received 2018-07-15, accepted for publication 2018-11-26, Published 2018-11-26
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摘要

Green tea is one of the most beverages with antioxidants and nutrients. As one of the major components of green tea, (-)-epicatechin gallate (ECG) was evaluated for its antioxidative properties in the present study. Cell proliferation assay, tube formation, cell migration, apoptosis, and autophagy were performed in human brain microvascular endothelial cells (HBMVECs) after oxygen-glucose deprivation/reoxygenation (OGD/R) to investigate potential anti-ischemia/reperfusion injury properties of ECG in vitro. Markers of oxidative stress as ROS, LDH, MDA, and SOD were further assayed in our study. Data indicated that ECG could affect neovascularization and promote cell proliferation, tube formation, and cell migration while inhibiting apoptosis and autophagy through affecting VEGF, Bcl-2, BAX, LC3B, caspase 3, mTOR, and Beclin-1 expression. All the data suggested that ECG may be protective for the brain against ischemia/reperfusion injury by promoting neovascularization, alleviating apoptosis and autophagy, and promoting cell proliferation in HBMVECs of OGD/R.

授权许可

Copyright © 2019 Bing Fu et al. 2019
This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

通讯作者

Min Li.Department of Neurology, The Second People’s Hospital of Lianyungang, Lianyungang, Jiangsu 222023, China, lygey.com.minlidudu@163.com

推荐引用方式

Bing Fu,Qinghong Zeng,Zhaoting Zhang,Mingyue Qian,Jiechun Chen,Wanli Dong,Min Li. Epicatechin Gallate Protects HBMVECs from Ischemia/Reperfusion Injury through Ameliorating Apoptosis and Autophagy and Promoting Neovascularization. Oxidative Medicine and Cellular Longevity ,Vol.2019(2019)

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