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Pharmaceutical Biology Volume 55 ,Issue 1 ,2017-01-01
Antioxidant activity against H2O2-induced cytotoxicity of the ethanol extract and compounds from Pyrola decorate leaves
Research Article
Xiliang Yang 1 Qingyun Peng 1 Qian Liu 1 Jie Hu 1 Zhipeng Tang 1 Lianjie Cui 1 Zonghao Lin 1 Bing Xu 1 Kuojian Lu 1 Fang Yang 1 Zhizheng Sheng 1 Qiong Yuan 1 Song Liu 1 Jiuliang Zhang 2 Xuefeng Zhou 3
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DOI:10.1080/13880209.2017.1333126
Received 2017-1-6, accepted for publication 2017-5-17, Published 2017-5-17
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摘要

Context: The leaves of Pyrola decorate H. Andr (Pyrolaceae), known as Luxiancao, have long been used for treating kidney deficiency, gastric haemorrhage and rheumatic arthritic diseases in traditional Chinese medicine.Objective: The phytochemicals and antioxidant capacities in vitro of P. decorate leaves were investigated.Materials and methods: Ethanol, petroleum ether, acetidin, n-butyl alcohol and aqueous extracts of Pyrola decorate leaves were prepared by solvent sequential process, and then isolated and purified to obtain phytochemicals. Cell viability was measured by MTT assay. PC12 cells were pretreated for 24 h with different extractions of P. decorate leaves at concentrations of 0.1, 0.5, 1, 5 and 10 mg/mL, then H2O2 of 0.4 mM was added in all samples for an additional 2 h. The antioxidant capacities of betulin, ursolic acid and monotropein were determined in PC12 cells against H2O2 induced cytotoxicity in vitro as well.Results: Nine compounds (1–9) were isolated and structurally determined by spectroscopic methods, especially 2D NMR analyses. Ethanol extract treated groups showed inhibitory activity with IC50 value of 10.83 mg/mL. Betulin, ursolic acid and monotropein were isolated from P. decorate, and demonstrated with IC50 values of 6.88, 6.15 and 6.13 μg/mL, respectively.Discussion and conclusions: In conclusion, Pyrola decorate is a potential antioxidative natural plant and worth testing for further pharmacological investigation in the treatment of oxidative stress related neurological disease.

关键词

isolation;oxidative stress;terpenoids;Pyrolaceae

授权许可

© 2017 The Author(s). Published by Informa UK Limited, trading as Taylor & Francis Group.
This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

图表

Chemical structures of compounds 1–9.

Protective effect of the alcohol extracts of Pyrola decorate on H2O2-induced cytotoxicity in cultured PC12 cells (means ± SD, n = 5). The data (cell viability, measured by MTT assay) were normalized and expressed as a percentage of the control group, which was set to 100%. Results were calculated from three independent experiments and are shown as mean ± SD. Compared with blank control group, #p < 0.05, ##p < 0.01; compared with H2O2 model group, *p < 0.05,**p < 0.01.

Protective effect of different polar extracts of Pyrola decorate on H2O2-induced cytotoxicity in cultured PC12 cells (means ± SD, n = 5). The data (cell viability, measured by MTT assay) were normalized and expressed as a percentage of the control group, which was set to 100%. Results were calculated from three independent experiments and are shown as mean ± SD. Compared with blank control group, #p < 0.05, ##p < 0.01; compared with H2O2 model group, *p < 0.05, **p < 0.01.

Protective effect of betulin (BE), ursolic acid (UA) and monotropein (MO), isolated from Pyrola decorate, on H2O2-induced cytotoxicity in cultured PC12 cells (means ± SD, n = 5). The data (cell viability, measured by MTT assay) were normalized and expressed as a percentage of the control group, which was set to 100%. Results were calculated from three independent experiments and are shown as mean ± SD. Compared with blank control group, #p < 0.05, ##p < 0.01; compared with H2O2 model group, *p < 0.05, **p < 0.01.

通讯作者

1. Xiliang Yang.Department of Pharmacy, Medical College, Wuhan University of Science and Technology, Wuhan, China.yxlyxl117@163.com
2. Xuefeng Zhou.Key Laboratory of Tropical Marine Bio-Resources and Ecology, Guangdong Key Laboratory of Marine Materia Medica, South China Sea Institute of Oceanology, Chinese Academy of Sciences, Guangzhou, Chin.xfzhou@scsio.ac.cn

推荐引用方式

Xiliang Yang,Qingyun Peng,Qian Liu,Jie Hu,Zhipeng Tang,Lianjie Cui,Zonghao Lin,Bing Xu,Kuojian Lu,Fang Yang,Zhizheng Sheng,Qiong Yuan,Song Liu,Jiuliang Zhang,Xuefeng Zhou. Antioxidant activity against H2O2-induced cytotoxicity of the ethanol extract and compounds from Pyrola decorate leaves. Pharmaceutical Biology ,Vol.55, Issue 1(2017)

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