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Journal of Chemistry Volume 2019 ,2019-01-16
Rapid Separation of All Four Tocopherol Homologues in Selected Fruit Seeds via Supercritical Fluid Chromatography Using a Solid-Core C18 Column
Research Article
Elise Urvaka 1 Inga Mišina 1 Arianne Soliven 2 , 3 Paweł Górnaś 1
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DOI:10.1155/2019/5307340
Received 2018-10-18, accepted for publication 2018-12-27, Published 2018-12-27
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摘要

Tocopherol separations employing the same Kinetex™ C18 column via supercritical fluid chromatography (SFC) and reversed-phase liquid chromatography (RP-LC) were compared. The application of the SFC system with UV diode array detection (DAD) resulted in rapid separation of all four tocopherol homologues with a total analysis time below 2 min. The RP-LC approach could not separate the isomers β and γ. The developed SFC-DAD method was precise, accurate, and most importantly more environmentally friendlier compared to the RP-LC method due to the 125-fold decrease in methanol consumption. The present study illustrated the selectivity differences between LC and SFC and how the C18 column can be used for tocopherol characterization. The optimized SFC method was successfully applied for the tocopherol determination in the seeds of nine different fruit species.

授权许可

Copyright © 2019 Elise Urvaka et al. 2019
This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

通讯作者

Paweł Górnaś.Institute of Horticulture, Latvia University of Life Sciences and Technologies, Graudu 1, Dobele LV-3701, Latvia.pavel.gornas@lvai.lv

推荐引用方式

Elise Urvaka,Inga Mišina,Arianne Soliven,Paweł Górnaś. Rapid Separation of All Four Tocopherol Homologues in Selected Fruit Seeds via Supercritical Fluid Chromatography Using a Solid-Core C18 Column. Journal of Chemistry ,Vol.2019(2019)

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