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Evidence-Based Complementray and Alternative Medicine Volume 2018 ,2018-10-18
Preparation of Magnetic Nanoliposomes of Sesquiterpene-Rich Fraction from Cichorium glandulosum and Its Tissue Distribution in Mice
Research Article
Yuqin Luo 1 , 2 Weijun Yang 3 Abudujilili Abuduaini 2 Haji Akber Aisa 1
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DOI:10.1155/2018/8549519
Received 2018-03-14, accepted for publication 2018-09-19, Published 2018-09-19
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摘要

Objective. To prepare and study the distribution of magnetic nanoliposomes of Sesquiterpene-Rich Fraction from Cichorium glandulosum (SRF-MLN) (under magnetic field) in the main organs of mice. Methods. The SRF-MLN was prepared by ethanol injection-sonication precipitation method. The quality of its pharmaceutical properties was investigated by the active ingredient lactucin. The drug concentration and distribution of lactucin in different tissues and organs including whole blood, liver, heart, spleen, lung, and kidney were evaluated with Sesquiterpene-Rich Fraction from Cichorium glandulosum (SRF-LP) as a control. Results. The prepared SRF-MLN was spherical and monodispersed with an average particle diameter of 65 ± 1 nm, encapsulation efficiency of 91.3%  ± 1.5% (RSD% = 1.5), and drug loading rate of 4.7%  ± 0.3% (RSD% = 0.7). Dispersion coefficient PDI was 0.157 and Zeta potential was −17.5 mV. The lactucin concentration of SRF-MLN after 10 min of intravenous injection in heart, liver, spleen, lung, and kidney was significantly higher than that of SRF-LP group. The AUC0-12h values of liver tissue were obviously higher than other tissues and MRT was significantly prolonged under the action of the magnetic field (p<0.01). Conclusion. The prepared SRF-MLN can change the distribution of drugs in different tissues and organs, prolonging the action time of the drugs in the body, and it has certain specificity under the action of applied magnetic field.

授权许可

Copyright © 2018 Yuqin Luo et al. 2018
This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

图表

Transmission electron microscopy of SRF-MLN.

SRF-LP distribution in various organs of mice.

SRF-MLN distribution in various organs of mice.

通讯作者

Haji Akber Aisa.Chinese Academy of Sciences Key Laboratory of Plant Resource and Natural Products Chemistry, Xinjiang Technical Institute of Physics and Chemistry, Chinese Academy of Sciences, Urumqi 830011, China, cas.cn.haji@ms.xjb.ac.cn

推荐引用方式

Yuqin Luo,Weijun Yang,Abudujilili Abuduaini,Haji Akber Aisa. Preparation of Magnetic Nanoliposomes of Sesquiterpene-Rich Fraction from Cichorium glandulosum and Its Tissue Distribution in Mice. Evidence-Based Complementray and Alternative Medicine ,Vol.2018(2018)

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参考文献
[1] A. D. Banghan, M. M. Standish, J. C. War kins. (1965). Diffusion of univalentions across the amellae of swollen phospholipid. Journal of Molecular Biology.13(2):228. DOI: 10.3181/00379727-146-38268.
[2] L. E. van Vlerken, Z. Duan, M. V. Seiden, M. M. Amiji. et al.(2007). Modulation of intracellular ceramide using polymeric nanoparticles to overcome multidrug resistance in cancer. Cancer Research.67(10):4843-4850. DOI: 10.3181/00379727-146-38268.
[3] H. K. Wu, Z. Su, A. Yili, Z. P. Xiao. et al.(2007). Isolation of esculetin from Cichorium glandulosum by high-speed countercurrent chromatography. Chemistry of Natural Compounds.43(1):109. DOI: 10.3181/00379727-146-38268.
[4] H. Q. Zhao, X. L. Xin, H. A. Aisa, Y. Y. Wei. et al.(2012). Screening Study on the Hypoglycemic Fraction from Cichorium glandulosum Boiss. et Huet. Lishizhen Medicine and Materiamedica Research:794-797. DOI: 10.3181/00379727-146-38268.
[5] Y. E. Rahman. (1974). The Challenge of Liposme Targeting in Vivo. Proceedings of the Society for Experimental Biology and Medicine.146(4):1173. DOI: 10.3181/00379727-146-38268.
[6] X. R. Wu, Liu. Z. H. L., S. H. C. H. Xin. (2008). In vivo distribution and therapeutic efficacy of magnetic liposomes containing paclitaxel for lung cancer in nude mice. China Pharmacv.19(30):2336. DOI: 10.3181/00379727-146-38268.
[7] H. Wu, Z. Su, Y. Yang, H. Ba. et al.(2007). Isolation of three sesquiterpene lactones from the roots of Cichorium glandulosum Boiss. et Huet.by high-speed counter-current chromatography. Journal of Chromatography A.1176(1-2):217-222. DOI: 10.3181/00379727-146-38268.
[8] X. L. Xin, H. K. Wu, Q. Y. Lv, H. A. Aisa. et al.(2012). Hypoglycemic effect of extracts from cichorium glandulosum. Natural Product Research and Development.24:234-238. DOI: 10.3181/00379727-146-38268.
[9] X. Xin, W. Yang, M. Yasen, H. Zhao. et al.(2014). The mechanism of hepatoprotective effect of sesquiterpene rich fraction from Cichorum glandulosum Boiss. Et Huet on immune reaction-induced liver injury in mice. Journal of Ethnopharmacology.155(2):1068-1075. DOI: 10.3181/00379727-146-38268.
[10] W.-Z. Yang, H. Wang, J. Shang, F. Feng. et al.(2009). Chemical constituents from Cichorium glandulosum. Chinese Journal of Natural Medicines.7(3):193-195. DOI: 10.3181/00379727-146-38268.
[11] X. Yao, L. Zhu, Y. Chen, J. Tian. et al.(2013). and antioxidant activity and -glucosidase, -amylase inhibitory effects of flavonoids from seeds. Food Chemistry.139(1–4):59-66. DOI: 10.3181/00379727-146-38268.
[12] D. D. Lasic. (1997). Recent developments in medical applications of liposomes: sterically stabilized liposomes in cancer therapy and gene delivery. Journal of Controlled Release.48(2-3):203-222. DOI: 10.3181/00379727-146-38268.
[13] V. A. Slepushkin, S. Simões, P. Dazin, M. S. Newman. et al.(1997). Sterically stabilized ph2 sensitive liposomes intracellular delivery of aqueous contents and prolonged circulation in vivo. The Journal of Biological Chemistry.272(4):2382-2388. DOI: 10.3181/00379727-146-38268.
[14] China Pharmacopoeia Committee. (2015). Pharmacopoeia of the People's Republic of China, The First Division of 2010 Edition. DOI: 10.3181/00379727-146-38268.
[15] China Pharmacopoeia Committee. (1998). Drug Standard of Ministry of Public Health of the Peoples Republic of China –Uygur Medicine Part. DOI: 10.3181/00379727-146-38268.
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