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eLife Volume 7 ,2018-06-30
TRPV4 is the temperature-sensitive ion channel of human sperm
Cell Biology
Nadine Mundt 1 , 2 Marc Spehr 2 Polina V Lishko 1
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DOI:10.7554/eLife.35853
Received 2018-02-11, accepted for publication 2018-06-30, Published 2018-06-30
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摘要

10.7554/eLife.35853.001Ion channels control the ability of human sperm to fertilize the egg by triggering hyperactivated motility, which is regulated by membrane potential, intracellular pH, and cytosolic calcium. Previous studies unraveled three essential ion channels that regulate these parameters: (1) the Ca2+ channel CatSper, (2) the K+ channel KSper, and (3) the H+ channel Hv1. However, the molecular identity of the sperm Na+ conductance that mediates initial membrane depolarization and, thus, triggers downstream signaling events is yet to be defined. Here, we functionally characterize DSper, the Depolarizing Channel of Sperm, as the temperature-activated channel TRPV4. It is functionally expressed at both mRNA and protein levels, while other temperature-sensitive TRPV channels are not functional in human sperm. DSper currents are activated by warm temperatures and mediate cation conductance, that shares a pharmacological profile reminiscent of TRPV4. Together, these results suggest that TRPV4 activation triggers initial membrane depolarization, facilitating both CatSper and Hv1 gating and, consequently, sperm hyperactivation.

关键词

Human;thermosensitivity;CatSper;hyperactivation;sperm cell;ion channel;TRPV4

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© 2018, Mundt et al
http://creativecommons.org/licenses/by/4.0/This article is distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use and redistribution provided that the original author and source are credited.

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Nadine Mundt,Marc Spehr,Polina V Lishko. TRPV4 is the temperature-sensitive ion channel of human sperm. eLife ,Vol.7(2018)

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