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Evidence-Based Complementray and Alternative Medicine Volume 2017 ,2017-05-17
Optimization of Extraction Process for Antidiabetic and Antioxidant Activities of Kursi Wufarikun Ziyabit Using Response Surface Methodology and Quantitative Analysis of Main Components
Research Article
Salamet Edirs 1 , 2 , 3 Ablajan Turak 1 , 2 Sodik Numonov 1 , 2 , 4 Xuelei Xin 1 , 2 Haji Akber Aisa 1 , 2
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DOI:10.1155/2017/6761719
Received 2017-02-25, accepted for publication 2017-04-26, Published 2017-04-26
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摘要

By using extraction yield, total polyphenolic content, antidiabetic activities (PTP-1B and α-glycosidase), and antioxidant activity (ABTS and DPPH) as indicated markers, the extraction conditions of the prescription Kursi Wufarikun Ziyabit (KWZ) were optimized by response surface methodology (RSM). Independent variables were ethanol concentration, extraction temperature, solid-to-solvent ratio, and extraction time. The result of RSM analysis showed that the four variables investigated have a significant effect (p<0.05) for Y1, Y2, Y3, Y4, and Y5 with R2 value of 0.9120, 0.9793, 0.9076, 0.9125, and 0.9709, respectively. Optimal conditions for the highest extraction yield of 39.28%, PTP-1B inhibition rate of 86.21%, α-glycosidase enzymes inhibition rate of 96.56%, and ABTS inhibition rate of 77.38% were derived at ethanol concentration 50.11%, extraction temperature 72.06°C, solid-to-solvent ratio 1 : 22.73 g/mL, and extraction time 2.93 h. On the basis of total polyphenol content of 48.44% in this optimal condition, the quantitative analysis of effective part of KWZ was characterized via UPLC method, 12 main components were identified by standard compounds, and all of them have shown good regression within the test ranges and the total content of them was 11.18%.

授权许可

Copyright © 2017 Salamet Edirs et al. 2017
This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

图表

Response surface plot for the effects of investigated parameters on the EY.

Response surface plot for the effects of investigated parameters on the TPC.

Response surface plot for the effects of investigated parameters on the PTP-1B inhibition rate.

Response surface plot for the effects of investigated parameters on the α-glucosidase inhibition rate.

Response surface plot for the effects of investigated parameters on the ABTS inhibition rate.

UPLC chromatograms of the KWZ and mixed standard.

通讯作者

1. Xuelei Xin.The Key Laboratory of Plant Resources and Chemistry of Arid Zone, Xinjiang Technical Institute of Physics and Chemistry, Chinese Academy of Sciences, Urumqi 830011, China, cas.cn;State Key Laboratory Basis of Xinjiang Indigenous Medicinal Plants Resource Utilization, Xinjiang Technical Institute of Physics and Chemistry, Chinese Academy of Sciences, Urumqi 830011, China, cas.cn.xinxl@ms.xjb.ac.cn
2. Haji Akber Aisa.The Key Laboratory of Plant Resources and Chemistry of Arid Zone, Xinjiang Technical Institute of Physics and Chemistry, Chinese Academy of Sciences, Urumqi 830011, China, cas.cn;State Key Laboratory Basis of Xinjiang Indigenous Medicinal Plants Resource Utilization, Xinjiang Technical Institute of Physics and Chemistry, Chinese Academy of Sciences, Urumqi 830011, China, cas.cn.haji@ms.xjb.ac.cn

推荐引用方式

Salamet Edirs,Ablajan Turak,Sodik Numonov,Xuelei Xin,Haji Akber Aisa. Optimization of Extraction Process for Antidiabetic and Antioxidant Activities of Kursi Wufarikun Ziyabit Using Response Surface Methodology and Quantitative Analysis of Main Components. Evidence-Based Complementray and Alternative Medicine ,Vol.2017(2017)

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参考文献
[1] A. R. Lomasney, C. Guillo, A. M. Sidebottom, M. G. Roper. et al.(2009). Optimization of capillary electrophoresis conditions for a glucagon competitive immunoassay using response surface methodology. Analytical and Bioanalytical Chemistry.394(1):313-319. DOI: 10.1016/j.jep.2013.02.007.
[2] K. B. Kobakhidze, M. D. Alaniya, D. N. Aneli. (1999). Phenolic compounds of Geranium pusillum. Chemistry of Natural Compounds.35(6):683. DOI: 10.1016/j.jep.2013.02.007.
[3] S. H. Hwang, S. H. Kwon, Z. Wang, T. H. Kim. et al.(2016). Optimization of extraction parameters of PTP1 (protein tyrosine phosphatase 1), inhibitory polyphenols, and anthocyanins from Zea mays L. using response surface methodology (RSM). BMC Complementary and Alternative Medicine.16(1, article 317). DOI: 10.1016/j.jep.2013.02.007.
[4] X. Zheng, X. Xu, C. Liu, Y. Sun. et al.(2013). Extraction characteristics and optimal parameters of anthocyanin from blueberry powder under microwave-assisted extraction conditions. Separation and Purification Technology.104(5):17-25. DOI: 10.1016/j.jep.2013.02.007.
[5] Y.-C. Yang, J. Li, Y.-G. Zu, Y.-J. Fu. et al.(2010). Optimisation of microwave-assisted enzymatic extraction of corilagin and geraniin from Geranium sibiricum Linne and evaluation of antioxidant activity. Food Chemistry.122(1):373-380. DOI: 10.1016/j.jep.2013.02.007.
[6] P. Z. Mašković, L. D. Diamanto, A. Cvetanović, M. Radojković. et al.(2016). Optimization of the extraction process of antioxidants from orange using response surface methodology. Food Analytical Methods.9(5):1436-1443. DOI: 10.1016/j.jep.2013.02.007.
[7] G. Horvath, S. Sirrs, S. Stockler, R. Salvarinova-Zivkovic. et al.(2013). Three years experience with dried blood spot -glucosidase screening for Pompe disease in British Columbia, Canada. BMC Musculoskeletal Disorders.14. DOI: 10.1016/j.jep.2013.02.007.
[8] B. A. Ishanqulova, M. U. Sharofova. (2004). Vliyanie sbora "Fitobet" na techenie eksperimentalnovo sakharnogo diabeta. part 4. DOI: 10.1016/j.jep.2013.02.007.
[9] X. Xu, B. Huangerhan. (2009). Flora of Kazakh. part 4. DOI: 10.1016/j.jep.2013.02.007.
[10] L. Xu, Y. Xi. (1996). Hypoglycemic activity and hypoglycemic mechanism of several kinds of traditional medicines. World Phytomedicine.11(4):166-168. DOI: 10.1016/j.jep.2013.02.007.
[11] E. Panzhinskiy, Y. Hua, B. Culver, J. Ren. et al.(2013). Endoplasmic reticulum stress upregulates protein tyrosine phosphatase 1B and impairs glucose uptake in cultured myotubes. Diabetologia.56(3):598-607. DOI: 10.1016/j.jep.2013.02.007.
[12] N. A. M. Saleh, Z. A. R. El-karemy, R. M. A. Mansour, A.-A. A. Fayed. et al.(1983). A chemosystematic study of some geraniaceae. Phytochemistry.22(11):2501-2505. DOI: 10.1016/j.jep.2013.02.007.
[13] S. G. Wubshet, Y. Tahtah, A. M. Heskes, K. T. Kongstad. et al.(2016). Identification of PTP1B and -Glucosidase Inhibitory Serrulatanes from Eremophila spp. by Combined use of Dual High-Resolution PTP1B and -Glucosidase Inhibition Profiling and HPLC-HRMS-SPE-NMR. Journal of Natural Products.79(4):1063-1072. DOI: 10.1016/j.jep.2013.02.007.
[14] S. Bakhtiyari, R. Meshkani, M. Taghikhani, B. Larijani. et al.(2010). Protein tyrosine phosphatase-1B (PTP-1B) knockdown improves palmitate-Induced insulin resistance in C2C12 skeletal muscle cells. Lipids.45(3):237-244. DOI: 10.1016/j.jep.2013.02.007.
[15] H. L. Yu, J. P. Gao, D. Wang. (2007). Effect of Geranium wilfordii Maxim on Gastric Ulcer Induced by Ethanol in Mice. Lishizhen Medicine and Materia Medica Research.18(4):874-875. DOI: 10.1016/j.jep.2013.02.007.
[16] F. S. Sharopov, I. S. Gulmurodov, W. N. Setzer. (2010). Essential oil composition of L. and L. growing wild in Tajikistan. Journal of Chemical and Pharmaceutical Research.2:284-290. DOI: 10.1016/j.jep.2013.02.007.
[17] D. Şöhretoğlu, M. K. Sakar, S. A. Sabuncuoğlu, H. Özgüneş. et al.(2009). Antioxidant galloylated flavonoids from Geranium tuberosum L. subsp. tuberosum. Turkish Journal of Chemistry.33(5):685-692. DOI: 10.1016/j.jep.2013.02.007.
[18] G. Kızıl, M. Kızıl, M. Yavuz, S. Emen. et al.(2008). Antioxidant activities of ethanol extracts of Hypericum triquetrifolium and Hypericum scabroides. Pharmaceutical Biology.46(4):231-242. DOI: 10.1016/j.jep.2013.02.007.
[19] X. Liu. (2012). Research Progress on Inhibitors of Protein Tyrosine Phosphatase-1B. Science and Technology Review.30(10):72-79. DOI: 10.1016/j.jep.2013.02.007.
[20] S. Mansur, R. Abdulla, A. Ayupbec, H. Aisa. et al.(2016). Chemical Fingerprint Analysis and Quantitative Analysis of Rosa rugosa by UPLC-DAD. Molecules.21(12):1754-1763. DOI: 10.1016/j.jep.2013.02.007.
[21] G. Sun, P. Ren, Y. Bi, K. Bi. et al.(2007). Fingerprints of ginkgo leaf extract and dipyridamole injection by double qualitative similarities and double quantitative similarities. Chinese Journal of Chromatography.25(4):518-523. DOI: 10.1016/j.jep.2013.02.007.
[22] Z. Liu, Z. Cheng, Q. He, B. Lin. et al.(2016). Secondary metabolites from the flower buds of Lonicera japonica and their in vitro anti-diabetic activities. Fitoterapia.110:44-51. DOI: 10.1016/j.jep.2013.02.007.
[23] H. Teng, Y. H. Choi. (2012). Optimization of extraction of bioactive alkaloids and antioxidants from rhizome coptidis (coptis chinensis franch) by using response surface methodology. Food Science and Biotechnology.21(3):799-806. DOI: 10.1016/j.jep.2013.02.007.
[24] J. S. Guo, S. X. Wang, X. Li, T. R. Zhu. et al.(1987). Studies on the antibacterial constituents of Geranium sibiricum L. Acta Pharmaceutica Sinica.22(1):28-32. DOI: 10.1016/j.jep.2013.02.007.
[25] W.-Y. Kang, Y.-L. Song, L. Zhang. (2011). -Glucosidase inhibitory and antioxidant properties and antidiabetic activity of Hypericum ascyron L.. Medicinal Chemistry Research.20(7):809-816. DOI: 10.1016/j.jep.2013.02.007.
[26] Y. t. Zhang, Q. Li, H. Xing, X. F. Lu. et al.(2013). Evaluation of antioxidant activity of ten compounds in different tea samples by means of an on-line HPLC-DPPH assay. Food Research International.53(2):847-856. DOI: 10.1016/j.jep.2013.02.007.
[27] H. J. Jeong, J.-H. Park, M.-J. Kim. (2016). Optimization of the extraction process for fermented Rhus verniciflua stokes using response surface methodology. Food Science and Biotechnology.25(1):179-184. DOI: 10.1016/j.jep.2013.02.007.
[28] C. S. Fodorea, L. Vlase, S. E. Leucuta, M. Tamas. et al.(2005). Phytochemical study on some polyphenols of Geranium pyrenaicum. Chemistry of Natural Compounds.41(4):400-403. DOI: 10.1016/j.jep.2013.02.007.
[29] S. J. Choi, J. K. Kim, J. M. Jang, K. H. Shin. et al.(2012). Rapid identification of the -glucosidase inhibitory compounds from Thunberg's Geranium (Geranium thunbergii Sieb. et Zucc.). Food Science and Biotechnology.21(4):987-996. DOI: 10.1016/j.jep.2013.02.007.
[30] O. O. Olubomehin, K. A. Abo, E. O. Ajaiyeoba. (2013). Alpha-amylase inhibitory activity of two species and rat model anti-diabetic activities of extracts and fractions. Journal of Ethnopharmacology.146(3):811-814. DOI: 10.1016/j.jep.2013.02.007.
[31] L. Jiang, S. Numonov, K. Bobakulov, M. N. Qureshi. et al.(2015). Phytochemical profiling and evaluation of pharmacological activities of hypericum scabrum L. Molecules.20(6):11257-11271. DOI: 10.1016/j.jep.2013.02.007.
[32] China Pharmacopoeia Committee. (2015). Pharmacopoeia of the People's Republic of China, part 1. DOI: 10.1016/j.jep.2013.02.007.
[33] Y. H. Huang, G. D. Huang, M. Huang, C. X. Wang. et al.(2009). Therapeutical effect of Geranium sibirium extract on low rectal carcinoma. Chinese Traditional Patent Medicine.31(8):1161-1164. DOI: 10.1016/j.jep.2013.02.007.
[34] E. A. H. Mohamed, M. J. A. Siddiqui, L. F. Ang, A. Sadikun. et al.(2012). Potent -glucosidase and -amylase inhibitory activities of standardized 50
[35] ethanolic extracts and sinensetin from Benth as anti-diabetic mechanism. BMC Complementary and Alternative Medicine.12, article 176(1). DOI: 10.1016/j.jep.2013.02.007.
[36] N. Kumar, P. Bhandari, B. Singh, A. P. Gupta. et al.(2008). Reversed phase-HPLC for rapid determination of polyphenols in flowers of rose species. Journal of Separation Science.31(2):262-267. DOI: 10.1016/j.jep.2013.02.007.
[37] S.-H. Lee, M.-H. Park, J.-S. Han, Y. Jeong. et al.(2012). Bioactive compounds extracted from Gamtae (Ecklonia cava) by using enzymatic hydrolysis, a potent -glucosidase and -amylase inhibitor, alleviates postprandial hyperglycemia in diabetic mice. Food Science and Biotechnology.21(4):1149-1155. DOI: 10.1016/j.jep.2013.02.007.
[38] X. Liu, Z. Z. Wu, K. Yang, H. Y. Ding. et al.(2013). Quantitative analysis combined with chromatographic fingerprint for comprehensive evaluation of Danhong injection using HPLC-DAD. Journal of Pharmaceutical and Biomedical Analysis.76(6):70-74. DOI: 10.1016/j.jep.2013.02.007.
[39] Y. Q. Hu, D. L. Liu, Y. C. Zhou, Z. Y. Lei. et al.(2003). Study on anti-inflammatory and analgesic effects of Geranium Wilfordii. Northwest Pharmaceutical Journal.18(3):113-115. DOI: 10.1016/j.jep.2013.02.007.
[40] P. Gaitonde, P. Garhyan, C. Link, J. Y. Chien. et al.(2016). A Comprehensive Review of Novel Drug—Disease Models in Diabetes Drug Development. Clinical Pharmacokinetics.55(7):769-788. DOI: 10.1016/j.jep.2013.02.007.
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