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BioMed Research International Volume 2016 ,2016-12-15
Profiles of Inflammatory Cytokines in the Vitreous Fluid from Patients with Rhegmatogenous Retinal Detachment and Their Correlations with Clinical Features
Research Article
Shizuka Takahashi 1 Kobu Adachi 1 Yukihiko Suzuki 1 Atsuko Maeno 1 Mitsuru Nakazawa 1
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DOI:10.1155/2016/4256183
Received 2016-09-04, accepted for publication 2016-11-13, Published 2016-11-13
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摘要

Purpose. To characterize the profiles for inflammatory cytokines in the vitreous fluid from patients with rhegmatogenous retinal detachment (RRD) by comparing those of other vitreoretinal diseases and to analyze the correlation between intravitreal cytokines and clinical features. Materials and Methods. Vitreous fluid was obtained at the time of surgery from 28 RRD eyes. Vitreous fluid was similarly collected from patients with macular hole (MH), epiretinal membrane, proliferative diabetic retinopathy (PDR), and retinal vein occlusion as controls. Twenty-seven cytokines were measured. Intravitreal cytokine profiles in RRD were characterized by comparing these with those in other vitreoretinal diseases. We also analyzed the correlations between vitreous cytokines and clinical features. Results. There were statistical differences in the MCP-1, MIP-1β, and IP-10 between the RRD and MH, while the IL-6 and IL-8 exhibited levels that were between those for the PDR and MH. MIP-1β was significantly correlated to both the extent and duration of the RRD, while IL-8 was significantly correlated to the extent of the RRD. Conclusions. MCP-1, MIP-1β, and IP-10 may modify the pathologic features of RRD. The levels of these cytokines are related in part to the clinical features and the level of photoreceptor damage.

授权许可

Copyright © 2016 Shizuka Takahashi et al. 2016
This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

图表

Kruskal-Wallis test showed there were different elevations in the expression profiles of the intravitreal cytokines for RRD, MH, PDR, ERM, and RVO. Although there were significantly greater upregulations of MCP-1, MIP-1β, and IP-10 in RRD versus MH (Mann-Whitney U test, P<0.001, P=0.041, and P<0.001, resp.), there were no significant differences observed between RRD and PDR. IL-6 and IL-8 were significantly elevated to a greater degree in RRD versus MH (P=0.009, P=0.007, resp.), while they were significantly lower in RRD versus PDR (P=0.037, P<0.001, resp.). ERM and RVO data are not shown. ∗ indicates the cytokines which showed to be statistically significantly upregulated in the RRD vitreous compared with MH.

Significant correlations were observed between the IL-8 and the MCP-1 (a), MIP-1β (b), and IP-10 (c) in the RRD vitreous. R1 with a solid regression line indicates the correlation coefficient when the MH data are not included as a control.

Significant correlations were observed between the IL-8 and the MCP-1 (a), MIP-1β (b), and IP-10 (c) in the RRD vitreous. R1 with a solid regression line indicates the correlation coefficient when the MH data are not included as a control.

Significant correlations were observed between the IL-8 and the MCP-1 (a), MIP-1β (b), and IP-10 (c) in the RRD vitreous. R1 with a solid regression line indicates the correlation coefficient when the MH data are not included as a control.

Correlations observed between the extent of RD and the IL-6, IL-8, MCP-1, MIP-1β, and IP-10. R1 with a solid regression line indicates Pearson’s correlation coefficient when the MH data were not included, while R2 with a broken regression line indicates that the MH data were included as a detached area of the 0 quadrant.

Correlations observed between the extent of RD and the IL-6, IL-8, MCP-1, MIP-1β, and IP-10. R1 with a solid regression line indicates Pearson’s correlation coefficient when the MH data were not included, while R2 with a broken regression line indicates that the MH data were included as a detached area of the 0 quadrant.

Correlations observed between the extent of RD and the IL-6, IL-8, MCP-1, MIP-1β, and IP-10. R1 with a solid regression line indicates Pearson’s correlation coefficient when the MH data were not included, while R2 with a broken regression line indicates that the MH data were included as a detached area of the 0 quadrant.

Correlations observed between the extent of RD and the IL-6, IL-8, MCP-1, MIP-1β, and IP-10. R1 with a solid regression line indicates Pearson’s correlation coefficient when the MH data were not included, while R2 with a broken regression line indicates that the MH data were included as a detached area of the 0 quadrant.

Correlations observed between the extent of RD and the IL-6, IL-8, MCP-1, MIP-1β, and IP-10. R1 with a solid regression line indicates Pearson’s correlation coefficient when the MH data were not included, while R2 with a broken regression line indicates that the MH data were included as a detached area of the 0 quadrant.

Correlations observed between the RRD and the IL-6, IL-8, MCP-1, MIP-1β, and IP-10 with the duration of RRD. R1 with a solid regression line means Pearson’s correlation coefficient when data of MH are not included, while R2 with a broken regression line is that when the data of MH are included as a duration of the 0 week. While only R1 of MIP-1β exhibited a significant correlation to IL-8, statistical significance was observed for all of R2s.

Correlations observed between the RRD and the IL-6, IL-8, MCP-1, MIP-1β, and IP-10 with the duration of RRD. R1 with a solid regression line means Pearson’s correlation coefficient when data of MH are not included, while R2 with a broken regression line is that when the data of MH are included as a duration of the 0 week. While only R1 of MIP-1β exhibited a significant correlation to IL-8, statistical significance was observed for all of R2s.

Correlations observed between the RRD and the IL-6, IL-8, MCP-1, MIP-1β, and IP-10 with the duration of RRD. R1 with a solid regression line means Pearson’s correlation coefficient when data of MH are not included, while R2 with a broken regression line is that when the data of MH are included as a duration of the 0 week. While only R1 of MIP-1β exhibited a significant correlation to IL-8, statistical significance was observed for all of R2s.

Correlations observed between the RRD and the IL-6, IL-8, MCP-1, MIP-1β, and IP-10 with the duration of RRD. R1 with a solid regression line means Pearson’s correlation coefficient when data of MH are not included, while R2 with a broken regression line is that when the data of MH are included as a duration of the 0 week. While only R1 of MIP-1β exhibited a significant correlation to IL-8, statistical significance was observed for all of R2s.

Correlations observed between the RRD and the IL-6, IL-8, MCP-1, MIP-1β, and IP-10 with the duration of RRD. R1 with a solid regression line means Pearson’s correlation coefficient when data of MH are not included, while R2 with a broken regression line is that when the data of MH are included as a duration of the 0 week. While only R1 of MIP-1β exhibited a significant correlation to IL-8, statistical significance was observed for all of R2s.

通讯作者

Mitsuru Nakazawa.Department of Ophthalmology, Hirosaki University Graduate School of Medicine, Hirosaki, Japan, hirosaki-u.ac.jp.mitsuru@hirosaki-u.ac.jp

推荐引用方式

Shizuka Takahashi,Kobu Adachi,Yukihiko Suzuki,Atsuko Maeno,Mitsuru Nakazawa. Profiles of Inflammatory Cytokines in the Vitreous Fluid from Patients with Rhegmatogenous Retinal Detachment and Their Correlations with Clinical Features. BioMed Research International ,Vol.2016(2016)

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