首页 » 文章 » 文章详细信息
Bioinorganic Chemistry and Applications Volume 2016 ,2016-02-10
Synthesis, Crystal Structure, Spectroscopic Properties, and Interaction with Ct-DNA of Zn(II) with 2-Aminoethanethiol Hydrochloride Ligand
Research Article
Xu-gang Shu 1 Chun-li Wu 1 Cui-jin Li 1 Min Zhang 2 Ke Wan 2 Xin Wu 3
Show affiliations
DOI:10.1155/2016/2691253
Received 2015-11-16, accepted for publication 2016-01-14, Published 2016-01-14
PDF
摘要

The zinc(II) complex (C2H6NS)2Zn·ZnCl2 was synthesized with 2-aminoethanethiol hydrochloride and zinc sulfate heptahydrate as the raw materials in aqueous solution. The composition and structure of the complex were characterized by elemental analysis, infrared spectra, single crystal X-ray diffraction, and thermogravimetry. The crystal structure of the zinc(II) complex belongs to monoclinic system, space group P  21/n, with cell parameters of a = 0.84294(4), b = 0.83920(4), c = 1.65787(8) nm, Z=2, and D = 2.041 g/cm3. In this paper, the interaction of complex with Ct-DNA was investigated by UV-visible and viscosimetric techniques. Upon addition of the complex, important changes were observed in the characteristic UV-Vis bands (hyperchromism) of calf thymus DNA and some changes in specific viscosity. The experimental results showed that the complex is bound to DNA intercalative (intercalation binding).

授权许可

Copyright © 2016 Xu-gang Shu et al. 2016
This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

图表

The molecular structure of compound 1 (ellipsoid parameter(s) 30%).

Crystal packing diagram of compound 1 (dotted lines indicate H bonding).

Crystal packing diagram of compound 1 (dotted lines indicate H bonding).

The standard FT-IR spectrum of 2-aminoethanethiol hydrochloride.

FT-IR spectrum of the complex.

The process of the reaction.

Thermogravimetric curve of the complex.

The UV-Vis absorption spectra of complex (1.7 × 10−4 mol/L) with Ct-DNA (from bottom to top, 0, 2 × 10−5 mol/L, 2.8 × 10−5 mol/L, 3.6 × 10−5 mol/L, 4.4 × 10−5 mol/L, 5.2 × 10−5 mol/L, and 6 × 10−5 mol/L).

Effect of increasing amount of the complex on viscosities of Ct-DNA at 25°C ([Complex]/[DNA] = 2.91, 3.35, 3.96, 4.84, 6.23, and 8.72).

通讯作者

1. Xu-gang Shu.College of Chemistry and Chemical Engineering, Zhongkai University of Agriculture and Engineering, Guangzhou 510225, China, zhku.edu.cn.xgshu@21cn.com
2. Xin Wu.Key Laboratory of Agro-Ecological Processes in Subtropical Region, Institute of Subtropical Agriculture, Chinese Academy of Sciences, Changsha 410125, China, cas.cn.wuxin@isa.ac.cn

推荐引用方式

Xu-gang Shu,Chun-li Wu,Cui-jin Li,Min Zhang,Ke Wan,Xin Wu. Synthesis, Crystal Structure, Spectroscopic Properties, and Interaction with Ct-DNA of Zn(II) with 2-Aminoethanethiol Hydrochloride Ligand. Bioinorganic Chemistry and Applications ,Vol.2016(2016)

您觉得这篇文章对您有帮助吗?
分享和收藏
0

是否收藏?

参考文献
[1] G. Predieri, D. Beltrami, R. Pattacini, M. L. Parisi. et al.(2009). Structural studies in solution and in the solid state on the zinc chelate of 2-hydroxy-(4-methylthio)butanoic acid, an effective mineral supplement in animal feeding. Inorganica Chimica Acta.362(4):1115-1121. DOI: 10.1038/emm.2004.74.
[2] Y. Li, X. Liu, Y. Zhang, X. Ma. et al.(2013). Effects of cysteamine on mRNA levels of growth hormone and its receptors and growth in orange-spotted grouper (). Fish Physiology and Biochemistry.39(3):605-613. DOI: 10.1038/emm.2004.74.
[3] A. A. Jamali, A. Tavakoli, J. E. N. Dolatabadi. (2012). Analytical overview of DNA interaction with Morin and its metal complexes. European Food Research and Technology.235(3):367-373. DOI: 10.1038/emm.2004.74.
[4] N. Shahabadi, S. Kashanian, K. Shalmashi, H. Roshanfekr. et al.(2009). DNA interaction with PtCl(LL) (LL = Chelating diamine ligand: -dimethyltrimethylendiamine) complex. Applied Biochemistry and Biotechnology.158(1):1-10. DOI: 10.1038/emm.2004.74.
[5] G. Elamaran, K. P. Singh, M. K. Singh, S. K. Singla. et al.(2012). Oxygen concentration and cysteamine supplementation during in vitro production of buffalo () embryos affect mRNA expression of BCL-2, BCL-XL, MCL-1, BAX and BID. Reproduction in Domestic Animals.47(6):1027-1036. DOI: 10.1038/emm.2004.74.
[6] C. Berruyer, F. M. Martin, R. Castellano, A. Macone. et al.(2004). Vanin-1 mice exhibit a glutathione-mediated tissue resistance to oxidative stress. Molecular and Cellular Biology.24(16):7214-7224. DOI: 10.1038/emm.2004.74.
[7] H. E. Mukaya, E. W. Neuse, R. L. Van Zyl, C. T. Chen. et al.(2015). Synthesis and preliminary bio-evaluation of polyaspartamide Co-conjugates of -amino-salicylic acid chelated platinum(II) and ferrocene complexes. Journal of Inorganic and Organometallic Polymers and Materials.25(3):367-375. DOI: 10.1038/emm.2004.74.
[8] A. Kutiyanawalla, W. Promsote, A. Terry, A. Pillai. et al.(2012). Cysteamine treatment ameliorates alterations in GAD67 expression and spatial memory in heterozygous reeler mice. International Journal of Neuropsychopharmacology.15(8):1073-1086. DOI: 10.1038/emm.2004.74.
[9] T. Balakrishnan, K. Ramamurthi. (2008). Growth, structural, optical, thermal and mechanical properties of glycine zinc chloride single crystal. Materials Letters.62(1):65-68. DOI: 10.1038/emm.2004.74.
[10] J.-H. Jeon, H.-J. Lee, G.-Y. Jang, C.-W. Kim. et al.(2004). Different inhibition characteristics of intracellular transglutaminase activity by cystamine and cysteamine. Experimental and Molecular Medicine.36(6):576-581. DOI: 10.1038/emm.2004.74.
[11] M. Lesort, M. Lee, J. Tucholski, G. V. W. Johnson. et al.(2003). Cystamine inhibits caspase activity: implications for the treatment of polyglutamine disorders. The Journal of Biological Chemistry.278(6):3825-3830. DOI: 10.1038/emm.2004.74.
[12] C. Wu, X. Shu, F. Chen. (2015). Synthesis and property analysis of cysteamine complex with zinc(II). Journal of Zhongkai University of Agriculture and Engineering.28(3):36-39. DOI: 10.1038/emm.2004.74.
[13] M. M. Shoukry, E. M. Khairy, A. A. El-Sherif. (2002). Ternary complexes involving copper(II) and amino acids, peptides and DNA constituents. The kinetics of hydrolysis of -amino acid esters. Transition Metal Chemistry.27(6):656-664. DOI: 10.1038/emm.2004.74.
[14] S.-M. Lee, E. M. Jeong, J. Jeong, D.-M. Shin. et al.(2012). Cysteamine prevents the development of lens opacity in a rat model of selenite-induced cataract. Investigative Ophthalmology & Visual Science.53(3):1452-1459. DOI: 10.1038/emm.2004.74.
[15] T. Balakrishnan, K. Ramamurthi. (2007). Structural, thermal and optical properties of a semiorganic nonlinear optical single crystal: glycine zinc sulphate. Spectrochimica Acta—Part A: Molecular and Biomolecular Spectroscopy.68(2):360-363. DOI: 10.1038/emm.2004.74.
[16] A. Najafi, H. D. Kia, H. Mohammadi, M. H. Najafi. et al.(2014). Different concentrations of cysteamine and ergothioneine improve microscopic and oxidative parameters in ram semen frozen with a soybean lecithin extender. Cryobiology.69(1):68-73. DOI: 10.1038/emm.2004.74.
[17] T. Zimmermann, J. V. Burda. (2010). Cisplatin interaction with amino acids cysteine and methionine from gas phase to solutions with constant pH. Interdisciplinary Sciences: Computational Life Sciences.2(1):98-114. DOI: 10.1038/emm.2004.74.
[18] D. M. Okamura, N. M. Bahrami, S. Ren, K. Pasichnyk. et al.(2014). Cysteamine modulates oxidative stress and blocks myofibroblast activity in CKD. Journal of the American Society of Nephrology.25(1):43-54. DOI: 10.1038/emm.2004.74.
文献评价指标
浏览 452次
下载全文 76次
评分次数 0次
用户评分 0.0分
分享 0次